Determination of glutathione redox potential and pH value in subcellular compartments of malaria parasites.
Identifieur interne : 000376 ( Main/Exploration ); précédent : 000375; suivant : 000377Determination of glutathione redox potential and pH value in subcellular compartments of malaria parasites.
Auteurs : Franziska Mohring [Allemagne] ; Mahsa Rahbari [Allemagne] ; Bernd Zechmann [États-Unis] ; Stefan Rahlfs [Allemagne] ; Jude M. Przyborski [Allemagne] ; Andreas J. Meyer [Allemagne] ; Katja Becker [Allemagne]Source :
- Free radical biology & medicine [ 1873-4596 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Anopheles (parasitologie), Antipaludiques (métabolisme), Antipaludiques (usage thérapeutique), Apicoplastes (métabolisme), Concentration en ions d'hydrogène (MeSH), Glutarédoxines (génétique), Glutarédoxines (métabolisme), Glutathion (génétique), Glutathion (métabolisme), Humains (MeSH), Interactions hôte-parasite (génétique), Mitochondries (métabolisme), Oxydoréduction (MeSH), Paludisme à Plasmodium falciparum (métabolisme), Paludisme à Plasmodium falciparum (parasitologie), Paludisme à Plasmodium falciparum (traitement médicamenteux), Plasmodium falciparum (génétique), Plasmodium falciparum (métabolisme), Plasmodium falciparum (pathogénicité), Protéines à fluorescence verte (métabolisme), Stress oxydatif (génétique).
- MESH :
- génétique : Glutarédoxines, Glutathion, Interactions hôte-parasite, Plasmodium falciparum, Stress oxydatif.
- métabolisme : Antipaludiques, Apicoplastes, Glutarédoxines, Glutathion, Mitochondries, Paludisme à Plasmodium falciparum, Plasmodium falciparum, Protéines à fluorescence verte.
- parasitologie : Anopheles, Paludisme à Plasmodium falciparum.
- pathogénicité : Plasmodium falciparum.
- traitement médicamenteux : Paludisme à Plasmodium falciparum.
- usage thérapeutique : Antipaludiques.
- Animaux, Concentration en ions d'hydrogène, Humains, Oxydoréduction.
English descriptors
- KwdEn :
- Animals (MeSH), Anopheles (parasitology), Antimalarials (metabolism), Antimalarials (therapeutic use), Apicoplasts (metabolism), Glutaredoxins (genetics), Glutaredoxins (metabolism), Glutathione (genetics), Glutathione (metabolism), Green Fluorescent Proteins (metabolism), Host-Parasite Interactions (genetics), Humans (MeSH), Hydrogen-Ion Concentration (MeSH), Malaria, Falciparum (drug therapy), Malaria, Falciparum (metabolism), Malaria, Falciparum (parasitology), Mitochondria (metabolism), Oxidation-Reduction (MeSH), Oxidative Stress (genetics), Plasmodium falciparum (genetics), Plasmodium falciparum (metabolism), Plasmodium falciparum (pathogenicity).
- MESH :
- chemical , genetics : Glutaredoxins, Glutathione.
- chemical , metabolism : Antimalarials, Glutaredoxins, Glutathione, Green Fluorescent Proteins.
- drug therapy : Malaria, Falciparum.
- genetics : Host-Parasite Interactions, Oxidative Stress, Plasmodium falciparum.
- metabolism : Apicoplasts, Malaria, Falciparum, Mitochondria, Plasmodium falciparum.
- parasitology : Anopheles, Malaria, Falciparum.
- pathogenicity : Plasmodium falciparum.
- chemical , therapeutic use : Antimalarials.
- Animals, Humans, Hydrogen-Ion Concentration, Oxidation-Reduction.
Abstract
The malaria parasite Plasmodium falciparum is exposed to multiple sources of oxidative challenge during its complex life cycle in the Anopheles vector and its human host. In order to further elucidate redox-based parasite host cell interactions and mechanisms of drug action, we targeted the genetically encoded glutathione redox sensor roGFP2 coupled to human glutaredoxin 1 (roGFP2-hGrx1) as well as the ratiometric pH sensor pHluorin to the apicoplast and the mitochondrion of P. falciparum. Using live cell imaging, this allowed for the first time the determination of the pH values of the apicoplast (7.12±0.40) and mitochondrion (7.37±0.09) in the intraerythrocytic asexual stages of the parasite. Based on the roGFP2-hGrx1 signals, glutathione-dependent redox potentials of -267mV and -328mV, respectively, were obtained. Employing these novel tools, initial studies on the effects of redox-active agents and clinically employed antimalarial drugs were carried out on both organelles.
DOI: 10.1016/j.freeradbiomed.2017.01.001
PubMed: 28062360
Affiliations:
- Allemagne, États-Unis
- District de Cologne, District de Giessen, Hesse (Land), Rhénanie-du-Nord-Westphalie, Texas
- Bonn, Marbourg
Links toward previous steps (curation, corpus...)
Le document en format XML
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Meyer</name><affiliation wicri:level="3"><nlm:affiliation>INRES-Chemical Signalling, University of Bonn, Friedrich-Ebert-Allee 144, 53113 Bonn, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>INRES-Chemical Signalling, University of Bonn, Friedrich-Ebert-Allee 144, 53113 Bonn</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Bonn</settlement></placeName></affiliation></author><author><name sortKey="Becker, Katja" sort="Becker, Katja" uniqKey="Becker K" first="Katja" last="Becker">Katja Becker</name><affiliation wicri:level="1"><nlm:affiliation>Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: katja.becker@uni-giessen.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen</wicri:regionArea><wicri:noRegion>35392 Giessen</wicri:noRegion><wicri:noRegion>35392 Giessen</wicri:noRegion><wicri:noRegion>35392 Giessen</wicri:noRegion></affiliation></author></analytic><series><title level="j">Free radical biology & medicine</title><idno type="eISSN">1873-4596</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Anopheles (parasitology)</term><term>Antimalarials (metabolism)</term><term>Antimalarials (therapeutic use)</term><term>Apicoplasts (metabolism)</term><term>Glutaredoxins (genetics)</term><term>Glutaredoxins (metabolism)</term><term>Glutathione (genetics)</term><term>Glutathione (metabolism)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Host-Parasite Interactions (genetics)</term><term>Humans (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Malaria, Falciparum (drug therapy)</term><term>Malaria, Falciparum (metabolism)</term><term>Malaria, Falciparum (parasitology)</term><term>Mitochondria (metabolism)</term><term>Oxidation-Reduction (MeSH)</term><term>Oxidative Stress (genetics)</term><term>Plasmodium falciparum (genetics)</term><term>Plasmodium falciparum (metabolism)</term><term>Plasmodium falciparum (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Anopheles (parasitologie)</term><term>Antipaludiques (métabolisme)</term><term>Antipaludiques (usage thérapeutique)</term><term>Apicoplastes (métabolisme)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Glutarédoxines (génétique)</term><term>Glutarédoxines (métabolisme)</term><term>Glutathion (génétique)</term><term>Glutathion (métabolisme)</term><term>Humains (MeSH)</term><term>Interactions hôte-parasite (génétique)</term><term>Mitochondries (métabolisme)</term><term>Oxydoréduction (MeSH)</term><term>Paludisme à Plasmodium falciparum (métabolisme)</term><term>Paludisme à Plasmodium falciparum (parasitologie)</term><term>Paludisme à Plasmodium falciparum (traitement médicamenteux)</term><term>Plasmodium falciparum (génétique)</term><term>Plasmodium falciparum (métabolisme)</term><term>Plasmodium falciparum (pathogénicité)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Stress oxydatif (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutaredoxins</term><term>Glutathione</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antimalarials</term><term>Glutaredoxins</term><term>Glutathione</term><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Malaria, Falciparum</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Host-Parasite Interactions</term><term>Oxidative Stress</term><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glutarédoxines</term><term>Glutathion</term><term>Interactions hôte-parasite</term><term>Plasmodium falciparum</term><term>Stress oxydatif</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Apicoplasts</term><term>Malaria, Falciparum</term><term>Mitochondria</term><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antipaludiques</term><term>Apicoplastes</term><term>Glutarédoxines</term><term>Glutathion</term><term>Mitochondries</term><term>Paludisme à Plasmodium falciparum</term><term>Plasmodium falciparum</term><term>Protéines à fluorescence verte</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Anopheles</term><term>Paludisme à Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Anopheles</term><term>Malaria, Falciparum</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Plasmodium falciparum</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Antimalarials</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Paludisme à Plasmodium falciparum</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Antipaludiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Oxidation-Reduction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Concentration en ions d'hydrogène</term><term>Humains</term><term>Oxydoréduction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The malaria parasite Plasmodium falciparum is exposed to multiple sources of oxidative challenge during its complex life cycle in the Anopheles vector and its human host. In order to further elucidate redox-based parasite host cell interactions and mechanisms of drug action, we targeted the genetically encoded glutathione redox sensor roGFP2 coupled to human glutaredoxin 1 (roGFP2-hGrx1) as well as the ratiometric pH sensor pHluorin to the apicoplast and the mitochondrion of P. falciparum. Using live cell imaging, this allowed for the first time the determination of the pH values of the apicoplast (7.12±0.40) and mitochondrion (7.37±0.09) in the intraerythrocytic asexual stages of the parasite. Based on the roGFP2-hGrx1 signals, glutathione-dependent redox potentials of -267mV and -328mV, respectively, were obtained. Employing these novel tools, initial studies on the effects of redox-active agents and clinically employed antimalarial drugs were carried out on both organelles.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28062360</PMID><DateCompleted><Year>2017</Year><Month>12</Month><Day>14</Day></DateCompleted><DateRevised><Year>2018</Year><Month>03</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-4596</ISSN><JournalIssue CitedMedium="Internet"><Volume>104</Volume><PubDate><Year>2017</Year><Month>03</Month></PubDate></JournalIssue><Title>Free radical biology & medicine</Title><ISOAbbreviation>Free Radic Biol Med</ISOAbbreviation></Journal><ArticleTitle>Determination of glutathione redox potential and pH value in subcellular compartments of malaria parasites.</ArticleTitle><Pagination><MedlinePgn>104-117</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0891-5849(17)30002-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.freeradbiomed.2017.01.001</ELocationID><Abstract><AbstractText>The malaria parasite Plasmodium falciparum is exposed to multiple sources of oxidative challenge during its complex life cycle in the Anopheles vector and its human host. In order to further elucidate redox-based parasite host cell interactions and mechanisms of drug action, we targeted the genetically encoded glutathione redox sensor roGFP2 coupled to human glutaredoxin 1 (roGFP2-hGrx1) as well as the ratiometric pH sensor pHluorin to the apicoplast and the mitochondrion of P. falciparum. Using live cell imaging, this allowed for the first time the determination of the pH values of the apicoplast (7.12±0.40) and mitochondrion (7.37±0.09) in the intraerythrocytic asexual stages of the parasite. Based on the roGFP2-hGrx1 signals, glutathione-dependent redox potentials of -267mV and -328mV, respectively, were obtained. Employing these novel tools, initial studies on the effects of redox-active agents and clinically employed antimalarial drugs were carried out on both organelles.</AbstractText><CopyrightInformation>Copyright © 2017 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mohring</LastName><ForeName>Franziska</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rahbari</LastName><ForeName>Mahsa</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zechmann</LastName><ForeName>Bernd</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Center for Microscopy and Imaging, Baylor University, 101 Bagby Ave., Waco, TX 76706, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rahlfs</LastName><ForeName>Stefan</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Przyborski</LastName><ForeName>Jude M</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Parasitology, Philipps University Marburg, Karl-von-Frisch Strasse 8, 35043 Marburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meyer</LastName><ForeName>Andreas J</ForeName><Initials>AJ</Initials><AffiliationInfo><Affiliation>INRES-Chemical Signalling, University of Bonn, Friedrich-Ebert-Allee 144, 53113 Bonn, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Becker</LastName><ForeName>Katja</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Biochemistry and Molecular Biology, Interdisciplinary Research Center, Justus Liebig University Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. Electronic address: katja.becker@uni-giessen.de.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>01</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Free Radic Biol Med</MedlineTA><NlmUniqueID>8709159</NlmUniqueID><ISSNLinking>0891-5849</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000962">Antimalarials</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C485187">PHluorin</NameOfSubstance></Chemical><Chemical><RegistryNumber>147336-22-9</RegistryNumber><NameOfSubstance UI="D049452">Green Fluorescent Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000852" MajorTopicYN="N">Anopheles</DescriptorName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000962" MajorTopicYN="N">Antimalarials</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000627" MajorTopicYN="N">therapeutic use</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D063546" MajorTopicYN="N">Apicoplasts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006790" MajorTopicYN="N">Host-Parasite Interactions</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016778" MajorTopicYN="N">Malaria, Falciparum</DescriptorName><QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000469" MajorTopicYN="N">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010963" MajorTopicYN="N">Plasmodium falciparum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Apicoplast</Keyword><Keyword MajorTopicYN="Y">Glutathione redox potential</Keyword><Keyword MajorTopicYN="Y">Mitochondrion</Keyword><Keyword MajorTopicYN="Y">Plasmodium falciparum</Keyword><Keyword MajorTopicYN="Y">pH</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>08</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>12</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>01</Month><Day>02</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>12</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28062360</ArticleId><ArticleId IdType="pii">S0891-5849(17)30002-3</ArticleId><ArticleId IdType="doi">10.1016/j.freeradbiomed.2017.01.001</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>États-Unis</li></country><region><li>District de Cologne</li><li>District de Giessen</li><li>Hesse (Land)</li><li>Rhénanie-du-Nord-Westphalie</li><li>Texas</li></region><settlement><li>Bonn</li><li>Marbourg</li></settlement></list><tree><country name="Allemagne"><noRegion><name sortKey="Mohring, Franziska" sort="Mohring, Franziska" uniqKey="Mohring F" first="Franziska" last="Mohring">Franziska Mohring</name></noRegion><name sortKey="Becker, Katja" sort="Becker, Katja" uniqKey="Becker K" first="Katja" last="Becker">Katja Becker</name><name sortKey="Meyer, Andreas J" sort="Meyer, Andreas J" uniqKey="Meyer A" first="Andreas J" last="Meyer">Andreas J. Meyer</name><name sortKey="Przyborski, Jude M" sort="Przyborski, Jude M" uniqKey="Przyborski J" first="Jude M" last="Przyborski">Jude M. Przyborski</name><name sortKey="Rahbari, Mahsa" sort="Rahbari, Mahsa" uniqKey="Rahbari M" first="Mahsa" last="Rahbari">Mahsa Rahbari</name><name sortKey="Rahlfs, Stefan" sort="Rahlfs, Stefan" uniqKey="Rahlfs S" first="Stefan" last="Rahlfs">Stefan Rahlfs</name></country><country name="États-Unis"><region name="Texas"><name sortKey="Zechmann, Bernd" sort="Zechmann, Bernd" uniqKey="Zechmann B" first="Bernd" last="Zechmann">Bernd Zechmann</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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